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Forschungsinstitut fuer Augenheilkunde
INSTITUTE FOR OPHTHALMIC RESEARCH
FORSCHUNGSINSTITUT FÜR AUGENHEILKUNDE

Methodology

  • Molecular genetics PCR
  • Sanger sequencing
  • Pyrosequencing
  • Next generation sequencing
  • High resolution melting technology and qPCR (Light cycler technology) and Taqman assays
  • Linkage analysis and Homozygosity mapping
  • Gene copy number analysis (ie. CGH - comparative genomic hybridization, qPCR)
  • RNA-Seq
  • Association studies
  • TALEN and CRISPR/Cas9 technology Molecular biology Eukaryotic Cell culture and Bacterial culture
  • Cloning
  • Heterologous expression of recombinant proteins
  • Heterologous splice assays, Enzymatic assays
  • Western blot
  • Immunohistochemistry
  • In situ hybridization
  • Yeast-two-hybrid analysis
  • Zebrafish manipulation, generation and characterization.