Services & Instrumentation
Metabolomics
Instrumentation
| LC systems | Ion sources | Mass spectrometers |
|---|---|---|
| Eksigent EKSPERT microLC 200 | ESI | ABSciex QTRAP 6500 |
| ExionLC UHPLC | ESI | ABSciex Triple Quad 4500 |
| Waters Acquity UPLC | ABSciex SelexIon (ion mobility) | Thermo Scientific LTQ Orbitrap XL |
| APCI |
Services
| Analytes | |
|---|---|
| Method development for targeted small molecule analysis | On demand and dependent on instrument availability |
| Biocrates AbsoluteIDQ® p180 Kit | Amino acids (Q), biogenic amines (Q), acetylcarnitines (SQ), sum of hexoses (SQ), sphingomyelins (SQ), phosphatidylcholines (SQ) |
| Chromsystems Kit | Amino acids (SQ) + acetylcarnithines (SQ) |
| Biocrates Bile Acid Kit | human and murine bile acids (Q) |
| Lipidomics screen | 300-400 lipid species (SQ) |
| Sphingolipids (under development) | sulfatides (Q), SM (Q), ceramide trihexosides (Q) |
- Q – quantitative
- SQ – semi-quantitative
Proteomics
Instrumentation
| LC systems | Instruments |
|---|---|
| 3x Dionex Ultimate 3000 RCLCnano | Thermo Scientific Orbitrap Fusion |
| Eksigent EKSPERT microLC 200 | Thermo Scientific QExactive Plus |
| Thermo Scientific LTQ Orbitrap Velos |
| ABSciex QTRAP 6500 |
Services
| Qualitative Proteomics | Quantitative Proteomics | Protein complex analysis |
|---|---|---|
| Shotgun proteomics | SILAC | Affinity-purification mass spectrometry (AP-MS) |
| Targeted (SRM, PRM) | Label-free quantification | quantitative AP-MS |
| Targeted (SRM, PRM) | Complex comparison (i.e. ± stimulus) |
Sample Preparation
Instrumentation
| Instrument |
|---|
| PBI Barocycler® NEP 2320 PCT SPS |
| Precellys Homogenizer |
| Tecan freedom EVO 100 Liquid handling system |
Services
- Protein digest (In solution, in gel, FASP, pressure-assisted)
- SDS-PAGE
- Isoelectric focusing (IEF)
- Extraction from very small sample amounts
- Metabolomics and proteomics of the identical sample (small sample amounts, e.g. biopsies)
- Processing of formalin-fixed, paraffin-embedded (FFPE) samples
- Processing of complex sample material, e.g. tissue samples, plasma (metabolomics), CSF, tear samples, urine